Iris Salmonella®

IRIS Salmonella® is an alternative research method of Salmonellae in human food and feeds, and environmental
sample (except primary production samples).

Studies performed on IRIS Salmonella® Agar show a high specificity for the detection of Salmonellae including
atypical species and serovars, which is a source of confusion on other medium.
Indeed, the detection of Salmonella Typhi and Paratyphi, lactose-positive Salmonellae (Salmonella Senftenberg and
subspecies S. arizonae and S. diarizonae), saccharose-positive strains are ensured.

The medium allows the detection of non-motile serovars (S. Pullorum and S. Gallinarum) or monophasic strains. IRIS
Salmonella® Agar allows also the detection of strains which show a light or absence of esterasic activity on other
medium (Salmonella bongori, Salmonella Dublin and Atento, certain strains of S. enterica, S. houtenae and S.
diarizonae subspecies).

The method is NF VALIDATION certified, with the IRIS Salmonella® supplement, according to the NF EN ISO 16140-
2 validation protocol of 2016 for the following categories:

– All food products (from 0 to 25 g)
– All animal feed and pet food (from 0 to 25 g), dry animal feed and pet food (from 50 g to 125 g)
– All samples from the industrial production environment
– Milk powders (including infant milk powders with and without probiotics) from 50 to 375 g

The method is also certified NF VALIDATION with the CSD supplement according to the validation protocol NF EN
ISO 16140-2 of 2016 for the following categories:

– Infant milk powders, with and without probiotics; ingredients for test intakes up to 50 g, with a 1/10th dilution
– Infant milk powders, with and without probiotics; ingredients for test intakes of 50-375 g, at 1/4 dilution
– Samples from the production environment

Refer to the certificate available on the NF VALIDATION website for the end date of validity of the method. The
reference method used for the validation is the standard NF EN ISO 6579-1 of 2017.

IRIS Salmonella® Agar may be used in the standard methods for the detection of Salmonellae as second isolation
medium.

The CSD supplement allows the common enrichment of Salmonella and Cronobacter in infant milk powders with
and without probiotics, ingredients, and environmental products.
Refer to the CSD method, BKR 23/12-12/20, certified NF VALIDATION, for the detection of Cronobacter spp

IRIS Salmonella Agar:
BM16008 – 20 plates (Ø 90 mm)
BM16108 – 120 plates (Ø 90 mm)

Salmonella Enrichment:
BK194HA – 500 g bottle
BK194GC – 5 kg drum
BM13608 – 10 x 225 mL vials
BM13708 – 3 x 3 L flexible bag
BM14408 – 2 x 5 L flexible bag
BM23708 – 40 x 5 L flexible bag

Salmonella Enrichment + Tween® 80:
BM16308 – 3 x 3 L flexible bag
BM19808 – 2 x 5 L flexible bag
BM22808 – 10 x 225 mL bottles

Salmonella Enrichment 9X Concentrated:
BM23308 – 4 x 2.5 L flexible bag

IRIS Salmonella Supplement:
BS07808 – 10 x 50 mL vials
BS07708 – 120 tablets qsp 225 mL
BS09308 – 120 tablets qsp 90 mL

IRIS Salmonella concentrate Supplement:
BS10208 – 10 bottles of 90 mL

Latex agglutination test:
BT01108 – CONFIRM’ Salmonella